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Contributor Information

  • Name Paul Crocker
  • Institute University of Dundee

Tool Details

  • Tool name: Siglec-F KO mouse
  • Alternate names: Siglec-8, Sialic Acid Binding Ig Like Lectin 8, SAF-2,
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Knock-Out
  • Conditional: No
  • Genetic background and cross history: Siglec FR114D knock in mice, carrying an Arg114 to Asp mutation in the Siglec F gene predicted to abolish sialic acid binding were generated by Taconic Artemis using C57Bl/6 ES cells (targeting strategy illustrated in PMID: 24698729). Further crossing of homozygous Siglec FR114D mice with Tg (Nes-Cre)1Wme/J(Bal1-Cre) mice produced offspring with mosaicism/partial deletion of exons 6-9 in adult organs (including germline) resulting in the truncated Siglec F knock out allele. Mosaic offspring were then used to generate Siglec F KO mice
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Description: Siglecs-8 and -F are paralogous membrane proteins highly expressed on human and mouse eosinophils respectively. They are members of the CD33-related sialic acid binding Ig-like lectin family and contain immunoreceptor tyrosine based inhibitory motifs (ITIM) and ITIM-like motifs in their cytoplasmic tails that are implicated in negative regulatory functions
  • Research area: Cell biology; Immunology
  • Production details: Siglec FR114D knock in mice, carrying an Arg114 to Asp mutation in the Siglec F gene predicted to abolish sialic acid binding were generated by Taconic Artemis using C57Bl/6 ES cells (targeting strategy illustrated in PMID: 24698729). Further crossing of homozygous Siglec FR114D mice with Tg (Nes-Cre)1Wme/J(Bal1-Cre) mice produced offspring with mosaicism/partial deletion of exons 6-9 in adult organs (including germline) resulting in the truncated Siglec F knock out allele. Mosaic offspring were then used to generate Siglec F KO mice

  • For Research Use Only

Target Details

  • Target: Siglec-F

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  McMillan et al. 2014. Immunol Lett. 160(1):11-16. PMID: 24698729.
  • •  Siglec-F-dependent negative regulation of allergen-induced eosinophilia depends critically on the experimental model.