Product Image

Contributor Information

  • Name Nic Jones
  • Institute Cancer Research UK, Manchester Institute

Tool Details

  • Tool name: ATF2 Flox Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Conditional KO
  • Conditional: Yes
  • Genetic background and cross history: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library, cloned into pPGKneobpAlox2PGKDlacZ-pA-neo-loxP, transfected into 129/ola embryonic stem cells and selected for G418 resistance. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
  • Phenotype: Homozygous normal phenotype
  • Zygosity: Homozygous
  • Description: Conditional knockout of ATF2 DNA binding domain. Transcription factor (BZIP-type, AP1-family)
  • Research area: Cell biology; Cell signaling and signal transduction; Developmental biology; Genetics; Immunology
  • Production details: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library, cloned into pPGKneobpAlox2PGKDlacZ-pA-neo-loxP, transfected into 129/ola embryonic stem cells and selected for G418 resistance. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.

  • For Research Use Only

Target Details

  • Target: ATF2

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

Related Tools

Product Image

Fzd96b_e1[22bp del] Zebrafish

#158356
Product Image

K5R2b Null Mouse

#151561
Product Image

UNG Mouse

#151556

References

  • •  Breitwieser et al. 2007. Genes Dev. 21(16):2069-82. PMID: 17699753.
  • •  Feedback regulation of p38 activity via ATF2 is essential for survival of embryonic liver cells.