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Contributor Information

  • Name Riccardo Dalla-Favera
  • Institute The Trustees of Columbia University in the City of New York

Tool Details

  • Tool name: MEF2BD83V Mouse
  • Alternate names: Myocyte Enhancer Factor 2B, Serum Response Factor-Like Protein 2, RSRFR2, XMEF2
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: B-Cell Lymphoma
  • Model: Knock-In
  • Conditional description: Conditional Knockin mice that express mutant MEF2BD38V upon recombination by Cre-recombinase
  • Genetic background and cross history: The conditional Mef2bstopD83V allele was generated by introducing a single nucleotide change by site directed mutagenesis leading to the D83V amino-acid change in exon 3. The neomycin-resistance marker followed by a triple-polyA, flanked by two loxP sites was introduced upstream the exon 3 carrying the D83V mutation. Correctly targeted mouse ES cells were injected into blastocysts derived from C57BL/6 mice to generate chimeras.
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Description: Diffuse large B-Cell Lymphoma (DLBCL) and Follicular Lymphoma (FL) are the two most common forms of mature B cell lymphoid neoplams, accounting for over 50% of all diagnoses. Mutant MEF2B (MEF2BD83V) is the most common lymphoma associated oncogene. MEF2BD83V expression in mice leads to GC enlargement and lymphoma development, a phenotype that becomes fully penetrant in combination with BCL2 de-regulation, an event associated with human MEF2B mutations. Expression of the mutant MEF2BD38V allele was induced in germinal centre- derived B cells by crossing with C1-Cre mice.
  • Research area: Cancer; Immunology
  • Production details: The conditional Mef2bstopD83V allele was generated by introducing a single nucleotide change by site directed mutagenesis leading to the D83V amino-acid change in exon 3. The neomycin-resistance marker followed by a triple-polyA, flanked by two loxP sites was introduced upstream the exon 3 carrying the D83V mutation. Correctly targeted mouse ES cells were injected into blastocysts derived from C57BL/6 mice to generate chimeras.

  • For Research Use Only

Target Details

  • Target: MEF2B

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Brescia et al. 2018. Cancer Cell. 34(3):453-465.e9. PMID: 30205047.
  • •  MEF2B Instructs Germinal Center Development and Acts as an Oncogene in B Cell Lymphomagenesis.