EXPERIMENTAL MODELS
Contributor Information
- Name Mike Owen
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: p23+/- Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Genetic background and cross history: A p23 targeting vector, constructed from genomic fragments, but replacing exon 1 of the p23 gene with a resistance marker, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the p23 knockout allele, p23+/- mice.
- Phenotype: Golgi apparatus abnormalities
- Zygosity: Heterozygous
- Strain: 129/C57BL/6
- Description: In vivo study of p23 knockout, golgi apparatus and early secretory pathway function
- Research area: Cancer; Cell signaling and signal transduction; Genetics; Immunology
- Production details: A p23 targeting vector, constructed from genomic fragments, but replacing exon 1 of the p23 gene with a resistance marker, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the p23 knockout allele, p23+/- mice.
- Additional notes: Genetic Bkg: 129/C57BL/6. Zygosity: Heterozygous
- For Research Use Only
Target Details
- Target: p23
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
References
- • Denzel et al. 2000. Curr Biol. 10(1):55-8. PMID: 10660306.
- • The p24 family member p23 is required for early embryonic development.
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