EXPERIMENTAL MODELS
Contributor Information
- Name Julian Lewis
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Jagged1 Floxed Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Cancer; Autoimmune disease
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Jag1 gene with loxP sites flanking exons 4 & 5 enabling conditional Cre-mediate knockout.
- Genetic background and cross history: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
- Phenotype:
- Zygosity: Heterozygous
- Description: Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
- Research area: Tissue-specific biology; Cell signaling and signal transduction; Genetics; Neurobiology
- Production details: A Jag1 targeting vector, containing a loxP flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL6 blastocyst. Chimeric offspring were mated on a C57BL6 background.
- Additional notes:
- For Research Use Only
Target Details
- Target: Jagged1 (Jag1)
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
Related Tools
References
- • Brooker et al. 2006. Development. 133(7):1277-86. PMID: 16495313.
- • Notch ligands with contrasting functions: Jagged1 and Delta1 in the mouse inner ear.
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