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Contributor Information

  • Name Steve Watson
  • Institute University of Birmingham

Tool Details

  • Tool name: LAIR-1 KO Mouse
  • Alternate names: Leukocyte-associated immunoglobulin-like receptor-1, LAIR-1, LAIR1, CD35, cluster of differentiation 35,
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Thrombocytosis
  • Model: Knock-Out
  • Conditional description: The proximal promoter and exons 1 and 2 of LAIR-1 were replaced with a flanked positive puromycin selection cassette expressed under the control of a eukaryotic promoter and includes a polyadenylation signal. Homologous recombinant clones were isolated using Puromycin resistance. The BAC targeting vector used was from the C57BL/6J RPCIB-731 BAC library and was transfected into the TaconicArtemis C57BL/6N Tac ES cell line. Removal of the promoter and exons 1 and 2 deletes the translation initiation codon and the sequence encoding the signal peptide. In addition the absence of the 5’UTR should also prevent transcription of the messenger RNA. Flp-mediated removal of the positive selection cassette also results in a constitutive KO allele.
  • Zygosity: Homozygous
  • Description: Leukocyte-associated immunoglobulin-like receptor-1, or LAIR-1, is a collagen receptor expressed on hematopoietic cells which can differentiate to peripheral mononuclear cells like NK, T and B cells. LAIR-1 is also expressed on immature megakaryocytes but undetectable on platelets, the physiological role that LAIR-1 plays on megakaryocytes and platelet formation is currently poorly understood. LAIR-1 belongs to the inhibitory immunoreceptor tyrosine-based inhibition motif–containing receptor family. The inhibitory receptor regulates the immune response preventing lysis of cells recognized as self. LAIR-1 inhibits signalling via the immunoreceptor tyrosine-based activation motif-containing receptor complex, glycoprotein VI-FcR-chain. The gene which encodes LAIR-1 is encoded in the leukocyte receptor cluster which also encodes at least 29 other genes encoding leukocyte-expressed receptors from the immunoglobulin superfamily. LAIR-1 is known to interact with PTPN11 and PTPN6.
  • Research area: Cell signaling and signal transduction; Immunology
  • Breeding information: The mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

  • For Research Use Only

Target Details

  • Target: Leukocyte-associated immunoglobulin-like receptor-1

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Smith et al. 2017. Arterioscler Thromb Vasc Biol. 37(5):823-835. PMID: 28336561.
  • •  Mice Lacking the Inhibitory Collagen Receptor LAIR-1 Exhibit a Mild Thrombocytosis and Hyperactive Platelets.