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Contributor Information

  • Name Richard Treisman
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: SAP1 Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Castleman disease;
  • Conditional: No
  • Conditional description:
  • Genetic background and cross history: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
  • Phenotype: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.
  • Zygosity:
  • Strain:
  • Description: In vivo study of SAP-1 knockout; in vivo study of positive T cell selection & development
  • Research area: Cell signaling and signal transduction; Genetics; Immunology
  • Production details: An Elk4 targeting vector, containing a resistance cassette inserted between exons 1 and 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologus recombination event were selected, cloned and injected into blastocysts. Chimeric offspring were backcrossed to establish heterozygous lines, and interbred to establish homozygous mice.
  • Additional notes: The SAP-1 mouse demonstrate a phenotype analogous to mutlicentric Castleman disease when maintained in non-sterile housing.SAP-1 mice exhibit abnormalities in T cell populations, resulting from abnormal negative selection during T cell development.

  • For Research Use Only

Target Details

  • Target: Elk4 / SAP-1

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Costello et al. 2004. Nat Immunol. 5(3):289-98. PMID: 14770179.
  • •  Ternary complex factor SAP-1 is required for Erk-mediated thymocyte positive selection.