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Contributor Information

  • Name Daniel Geraghty
  • Institute Fred Hutchinson Cancer Research Center
  • Primary citation lshitani et al. 2003. Journal of Immunology. 171(3):1376-84.PMID: 12874228

Tool Details

  • Tool name: Anti-HLA-E [4D12]
  • Clone: 4D12
  • Tool type: Antibody
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS, IP, Histology, Western Blot
  • Description: MoAb was combined to examine HLA expression in placental tissues for all the known forms of the nonclassical HLA-E class I molecules that is important in the maternal immune response during pregnancy. This MoAb provides a useful characteristic of binding to an epitope on the HLA-E complex that is distinct from MoAb 3D12 (Anti-HLA-E).
  • Isotype: IgG1
  • Research area: Immunology; Developmental biology

  • For Research Use Only

Target Details

  • Target: HLA-E nonclassical class I complexes in placental tissues
  • Target background: Antibody is used to examine expression in placental tissues for all the known forms of the HLA-E nonclassical class I molecules.

Application Details

  • Application: FACS, IP, Histology, Western Blot
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.

Documentation

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References

  • •  lshitani et al. 2003. Journal of Immunology. 171(3):1376-84.PMID: 12874228
  • •  Lee et al. 1998. J. Immunol.?160(10):4951-60. PMID: 9590243