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Contributor Information

  • Name Giampietro Schiavo
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Spastin [Sp 3G11/1]
  • Clone: Sp 3G11/1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse ; Rat
  • Host: Mouse
  • Molecular weight of the target: 52 kDa
  • Application: WB ; ChIP ; ELISA ; IHC ; IF ; WB
  • Strain: Balb/c
  • Description: Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein.
  • Immunogen: Recombinant human spastin
  • Isotype: IgG2a
  • Research area: Tissue-specific biology; Cell biology; Cell signaling and signal transduction; Genetics; Metabolism; Neurobiology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Spastin
  • Target molecular weight: 52 kDa
  • Target background: Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein.

Application Details

  • Application: WB ; ChIP ; ELISA ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Daftary et al. 2011. Mol Endocrinol. 25(9):1539-49. PMID: 21757506.
  • •  A novel role for the AAA ATPase spastin as a HOXA10 transcriptional corepressor in Ishikawa endometrial cells.
  • •  Lacroix et al. 2010. J Cell Biol. 189(6):945-54. PMID: 20530212.
  • •  Lacroix et al. 2010. J Cell Biol. 189(6):945-54. PMID: 20530212.
  • •  Tubulin polyglutamylation stimulates spastin-mediated microtubule severing.
  • •  Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033.
  • •  Human spastin has multiple microtubule-related functions.