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Contributor Information

  • Name Sibylle Mittnacht
  • Institute The Institute of Cancer Research

Tool Details

  • Tool name: Anti-Phospho-Rb (Ser608) [51B7]
  • Alternate names: Retinoblastoma 1; RB1; p15-Rb; RB; pp11; pRb; OSRC
  • Clone: 51B7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse
  • Host: Mouse
  • Cancer type: Breast cancer; Digestive / Gastrointestinal cancer; Genitourinary cancer; Head and neck cancer; Blood cancer
  • Molecular weight of the target: 111 kDa
  • Application: IHC ; IF ; WB
  • Description: Monoclonal antibody which detects the tumour suppressor Rb, when phosphorylated at residue Ser608.
  • Immunogen: Synthetic peptide C-ADMYLSPLRSPK phosphorylated at S608, conugated to KLH
  • Immunogen UniProt ID: P06400
  • Isotype: IgG1
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Immunology

  • For Research Use Only

Target Details

  • Target: Rb (phospho Ser608)
  • Target molecular weight: 111 kDa
  • Target background: Retinoblastoma protein (Rb) is a tumour suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. One function of Rb is to prevent excessive cell growth by inhibiting cell cycle progression. Rb is also a recruiter of several chromatin remodelling enzymes such as methylases and acetylases. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukaemia. Phosphorylation of Rb at S608 depends on CDK4.

Application Details

  • Application: IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Wadosky et al. 2016. J Mol Endocrinol. 56(3):273-90. PMID: 26862156.
  • •  Silva et al. 2014. PLoS One. 9(6):e99897. PMID: 24936870.
  • •  Repair of oxidative DNA damage, cell-cycle regulation and neuronal death may influence the clinical manifestation of Alzheimer's disease.
  • •  Shilkaitis et al. 2013. Cancer Prev Res (Phila). 6(4):299-308. PMID: 23430755.
  • •  Bettencourt-Dias et al. 2003. J Cell Sci. 116(Pt 19):4001-9. PMID: 12928330.
  • •  Barrie et al. 2003. Anal Biochem. 320(1):66-74. PMID: 12895470.
  • •  High-throughput screening for the identification of small-molecule inhibitors of retinoblastoma protein phosphorylation in cells.
  • •  Heterogeneous proliferative potential in regenerative adult newt cardiomyocytes.