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Contributor Information

  • Name Peter Stern
  • Institute Cancer Research UK, Manchester Institute

Tool Details

  • Tool name: Anti-5T4 [B1C3]
  • Alternate names: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
  • Clone: B1C3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mouse
  • Host: Mouse
  • Molecular weight of the target: 46.5 kDa
  • Application: ELISA ; FACS ; Fn
  • Strain: C57BL/6
  • Description: The murine 5T4 monoclonal antibody, clone B1C3, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.
  • Isotype: IgG2a
  • Research area: Cancer; Stem cell biology

  • For Research Use Only

Target Details

  • Target: 5T4 oncofetal glycoprotein
  • Target molecular weight: 46.5 kDa
  • Target background: The murine 5T4 monoclonal antibody, clone B1C3, defines the human 5T4 oncofoetal antigen, a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development.

Application Details

  • Application: ELISA ; FACS ; Fn

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.
  • •  CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.
  • •  Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.
  • •  The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
  • •  Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.
  • •  Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.