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Contributor Information

  • Name Margaret Goodall
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-CD74 [BU45]
  • Alternate names: HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Cluster of Differentiation 74
  • Clone: BU45
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Genitourinary cancer
  • Application: IHC
  • Strain: Balb/c
  • Description: CD74 is expressed primarily by antigen presenting cells such as B lymphocytes , macrophages and monocytes together, with many epithelialcells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing. CD74 is a type II transmembrane protein which binds to the binding groove of nacent MHC class II alpha/beta heterodimers preventing their premature association with endogenous polypeptides. The half life of CD74 on the cell surface is relatively short lasting only 3 to 4 min, before internalization. It has been suggested that CD74 on the cell surface binds MHC class II molecules which have lost their antigenic peptide promoting internalization and reloading with antigenic peptide.
  • Immunogen: B lymphoblastoid cell line: HFB1
  • Isotype: IgG1
  • Research area: Immunology

  • For Research Use Only

Target Details

  • Target: CD74
  • Target background: CD74 is expressed primarily by antigen presenting cells such as B lymphocytes , macrophages and monocytes together, with many epithelialcells. CD74 may exist in different isoforms ranging in size from 33 to 41kDa, depending on genetic splicing. CD74 is a type II transmembrane protein which binds to the binding groove of nacent MHC class II alpha/beta heterodimers preventing their premature association with endogenous polypeptides. The half life of CD74 on the cell surface is relatively short lasting only 3 to 4 min, before internalization. It has been suggested that CD74 on the cell surface binds MHC class II molecules which have lost their antigenic peptide promoting internalization and reloading with antigenic peptide.

Application Details

  • Application: IHC

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Leucocyte Typing IV, (1989): edited by W. Knapp, OUP, Oxford.
  • •  Leucocyte Typing V, (1995): edited by S.F. Schlossman, OUP, Oxford.
  • •  Wraight et al. 1990. J Biol Chem. 265(10):5787-92. PMID: 1690714.
  • •  Human major histocompatibility complex class II invariant chain is expressed on the cell surface.