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Contributor Information

  • Name Paul Crocker
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-SIGLEC3 [CD33 3D6]
  • Alternate names: Sialic Acid Binding Ig Like Lectin 12; SIGLECL1; SLG 3; Siglec-12; Siglec-L1; S2V
  • Clone: CD33 3D6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA
  • Description: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
  • Immunogen: CD33-Fc recombinant protein
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Immunology; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: SIGLEC3 (CD33)
  • Target background: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Application Details

  • Application: ELISA

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Taylor et al. 1999. J Biol Chem. 274(17):11505-12. PMID: 10206955.
  • •  The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2.
  • •  Freeman et al. 1995. Blood. 85(8):2005-12. PMID: 7718872.
  • •  Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules.