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Contributor Information

  • Name David Lane
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Rb1 [Rb1 (1F8)]
  • Clone: Rb1 (1F8)
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Eye cancer; Breast cancer;
  • Molecular weight of the target: 105 kDa
  • Application: IHC ; IF ; IP
  • Strain: Balb/c
  • Description: The Retinoblastoma protein (Rb) is a transcription factor that controls cell cycle progression (at the G1/S phase transition) through its repression of E2F-mediated transcription. Mutation of the Rb gene is found frequently in human sarcomas, lung, bladder and breast carcinomas and is the molecular basis for hereditary disposition to retinoblastoma.
  • Immunogen: Rb-b-galactosidase fusion protein spanning nucleotides 1126-1973 and representing 283 mino acids of Human Rb cDNA
  • Isotype: IgG1
  • Research area: Cancer; Cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Retinoblastoma (Rb1)
  • Target molecular weight: 105 kDa
  • Target background: The Retinoblastoma protein (Rb) is a transcription factor that controls cell cycle progression (at the G1/S phase transition) through its repression of E2F-mediated transcription. Mutation of the Rb gene is found frequently in human sarcomas, lung, bladder and breast carcinomas and is the molecular basis for hereditary disposition to retinoblastoma.

Application Details

  • Application: IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Antibodies targeting Clec9A promote strong humoral immunity without adjuvant in mice and non-human primates.
  •   Ragazzon et al. 2014. Eur J Endocrinol. 2014 Feb 4
  •   170(3):385-91. PMID: 24347427
  •   Bandyopadhyay et al. 2007. Aging Cell. 6(4):577-91. PMID: 17578512.
  •   Dynamic assembly of chromatin complexes during cellular senescence: implications for the growth arrest of human melanocytic nevi.
  •   Lee et al. 2007. Clin Cancer Res. 13(3):832-8. PMID: 17289874.
  •   Epigenetic inactivation of the chromosomal stability control genes BRCA1, BRCA2, and XRCC5 in non-small cell lung cancer.
  •   Bartkova et al. 2003. J Pathol. 200(2):149-56. PMID: 12754735.
  •   Deregulation of the RB pathway in human testicular germ cell tumours.
  •   Brtek et al. 1992. Oncogene. 7(1):101-8. PMID: 1741157.
  •   Cellular localization and T antigen binding of the retinoblastoma protein.
  •   Grand et al. 1989. Oncogene. 4(11):1291-8. PMID: 2682458.
  •   The expression of the retinoblastoma gene product Rb1 in primary and adenovirus-transformed human cells.