Product Image

Contributor Information

  • Name Maria E Teves Ph.D.
  • Institute Virginia Commonwealth University
  • Primary citation Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344

Tool Details

  • Tool name: Spag17 flox mouse
  • Tool type: Mouse
  • Disease: Developmental defects, primary cilia dyskinesia, accelerated aging, male infertility, and multi-organ fibrosis
  • Model: Knock-out
  • CRISPR: No
  • Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging
  • Genetic background and cross history: Spag17+/flox mice were mated with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice demonstrating a mixed background of C57BL/6J–129S4/SvJ
  • Phenotype: Multi-organ fibrosis
  • Zygosity: Homozygous
  • Strain: C57BL/6J wild-type females, 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J, and CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice
  • Description: Spag17 flox mouse to generate the Spag17 knockout mouse after Cre recombination. Spag17 is a complex gene associated with cilia and microtubule cytoskeleton. Loss of Spag17 leads to developmental defects, primary cilia dyskinesia, accelerated aging, male infertility, and multi-organ fibrosis. The novel flox mouse model that was created has the potential to generate a knockout for Spag17 once crossed with a Cre mouse.
  • Research area: Skeletal dysplasia; Cell biology; Primary cilia dyskinesia; Organ fibrosis
  • Production details: Embryonic stem cells with conditional potential were used to generate chimeric mice which were crossed to C57BL/6J wild-type females. Resulting mice were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J mice to remove the Neo cassette. The Spag17+/flox mice were used for mating with CMV-Cre (B6.C-Tg(CMV-cre)1Cgn/J) mice to generate Spag17+/?;CMV-Cre mice. Male and female Spag17+/?;CMV-Cre mice were mated, resulting in Mendelian inheritance for Spag17?/?;CMV-Cre mice. Afterwards, mice demonstrated a mixed background of C57BL/6J–129S4/SvJ.

  • For Research Use Only

Target Details

Application Details

  • Application: Southern blot, RT-PCR, western blotting, IHC, immunofluoresence, electron microscopy, and magnetic resonance imaging

Handling

Documentation

Related Tools

Product Image

CYFIP 1 [10bp insertion] Zebrafish

#158358
Product Image

HBRN Mouse

#151773
Product Image

Fibroblast specific Spag17 knockout mouse

#161683

References

  • •  Teves et al. Am J Respir Cell Mol Biol. 48(6):765-72. PMID: 23418344