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Contributor Information

  • Name Franz Radner
  • Institute University of Graz

Tool Details

  • Tool name: PNPLA1-flox mouse
  • Alternate names: PNPLA1
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Conditional KO
  • Conditional: Yes
  • Conditional description: Targeted conditional ready
  • Genetic background and cross history: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
  • Phenotype: These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
  • Zygosity: Homozygous
  • Strain: C57BL/6J
  • Description: Useful model for the generation of tissue/cell-specific PNPLA1-deficient mice. Animals might be useful for studies, e.g. on skin development/barrier function or omega-O-acylceramide metabolism, or as model for Autosomal Recessive Congenital Ichthyosis (ARCI).
  • Research area: Metabolism; Cell signaling and signal transduction
  • Production details: A floxed neomycin resistance cassette was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette and left Pnpla1 exon 1 floxed. Further details are available upon request.
  • Breeding information: Good breeder. Homozygous mice may be bred together to maintain a live colony,.

  • For Research Use Only

Target Details

  • Target: Patatin-like phospholipase domain containing 1, ichthyosis, ARCI

Application Details

Handling

Documentation

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References

  • •  Grond et al. 2017. J Invest Dermatol. 137(2):394-402. PMID: 27751867.