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Contributor Information

  • Name Franz Radner ; Rudolf Zechner
  • Institute University of Graz

Tool Details

  • Tool name: ABHD5-flox mouse
  • Alternate names: ABHD5, CGI-58
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Conditional KO
  • Conditional: Yes
  • Conditional description: Conditional ready
  • Genetic background and cross history: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are backcrossed to C57BL/6J regularly to avoid generation of subpopulations.
  • Phenotype: These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
  • Zygosity: Homozygous
  • Strain: C57BL/6J
  • Description: Useful model for the generation of tissue/cell specific ABHD5-deficient mice. Animals might be useful for studies, e.g. on energy metabolism or skin barrier development/function.
  • Research area: Metabolism; Developmental biology
  • Production details: A loxP site was inserted upstream of exon 4 and a floxed neomycin resistance cassette was inserted downstream of exon 7. Cre-mediated recombination removed the selection cassette and left Abhd5 exon 4 – 7 flanked by loxP sites. Further details are available upon request.
  • Breeding information: Good breeder. Homozygous mice may be bred together to maintain a live colony.

  • For Research Use Only

Target Details

  • Target: Abhydrolase domain containing 5, comparative gene identification-58, Chanarin-Dorfman, Ichthyosis

Application Details

Handling

Documentation

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References

  • •  23413028
  • •  Grond et al. 2017. J Invest Dermatol. 137(2):403-413. PMID: 27725204.
  • •  Jaeger et al. 2015. J Hepatol. 63(2):437-45. PMID: 25733154.
  • •  Radner et al. 2010. J Biol Chem. 285(10):7300-11. PMID: 20023287.