EXPERIMENTAL MODELS
Contributor Information
- Name Catrin Pritchard
- Institute University of Leicester
Tool Details
- Tool name: Raf KI D486A Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Cancer
- Conditional: No
- Cell signalling pathway: Ras/Raf signalling
- Genetic background and cross history: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
- Description: Knockin of oncogenic Raf-1D486A; in vivo study of oncogenic Raf-1 mutant, and Ras signalling
- Research area: Cancer; Cell signaling and signal transduction; Genetics; Neurobiology
- Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residue 486 (D to A) and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Raf-1 was targeted by homologous recombination with the targeting vector. Correctly targeted ES cells were transfected with a Cre expressing vector to excise the resistance cassette, before injection into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
- For Research Use Only
Target Details
- Target: Raf1 D486A (oncogenic mutant)
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
Related Tools
References
- • Kamata et al. 2010. Cancer Res. 70(21):8475-86. PMID: 20978199.
- • BRAF inactivation drives aneuploidy by deregulating CRAF.
- • Noble et al. 2008. Mol Cell. 31(6):862-72. PMID: 18922468.
- • CRAF autophosphorylation of serine 621 is required to prevent its proteasome-mediated degradation.
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