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Contributor Information

  • Name Jiang Chang
  • Institute Texas A&M University

Tool Details

  • Tool name: TG-aMHC-STOP-CD63NanoLuc mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Knock-In
  • Conditional: Yes
  • Conditional description: CD63Nan
  • Genetic background and cross history: In order to achieve a controllable exosome tagging, a dSTOP sequence flanked by two loxP sites was introduced upstream of CD63NanoLuc, and the expression cassette was driven by cardiomyocyte-specific Îą-MHC promoter (Fig). Linearized DNA containing ÎąMHC-STOP-CD63NanoLuc cassette was used for pronuclear microinjection to generate the founder of the transgenic strain.
  • Phenotype: Please see linked reference that details the generation and characterization of this mouse model.
  • Strain: C57BL/6
  • Description: This mouse models allows for researchers to track an visualize endogenous exosomes in real time. A NanoLuc reporter is fused to the exosome surface marker CD63 and expression is under control of a cardiomyocyte-specific promoter and conditional for tamoxifen.
  • Research area: Tissue-specific biology; Cell biology; Cell signaling and signal transduction
  • Additional notes: Please see linked reference that details the generation and characterization of this mouse model.

  • For Research Use Only

Target Details

  • Target: Exosomes expressing CD63

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Luo et al. 2020. Commun Biol. 3(1):114. PMID: 32157172.