EXPERIMENTAL MODELS
Contributor Information
- Name Nic Jones
- Institute Cancer Research UK, Manchester Institute
Tool Details
- Tool name: ATF2 Deleter Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Conditional: No
- Genetic background and cross history: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library (RZPD), cloned into pPGKneobpAlox2PGKD, transfected into 129/ola embryonic stem cells. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
- Phenotype: Homozygous postnatal lethality, heterozygous normal phenotype
- Zygosity: Heterozygous
- Description: Deletion of ATF2 DNA binding domain. Transcription factor (BZIP-type, AP1-family).
- Research area: Cell biology; Cell signaling and signal transduction; Developmental biology; Genetics; Immunology
- Production details: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library (RZPD), cloned into pPGKneobpAlox2PGKD, transfected into 129/ola embryonic stem cells. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
- For Research Use Only
Target Details
- Target: ATF2
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
References
- • Breitwieser et al. 2007. Genes Dev. 21(16):2069-82. PMID: 17699753.
- • Feedback regulation of p38 activity via ATF2 is essential for survival of embryonic liver cells.
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