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Contributor Information

  • Name Robert Zimmermann ; Franz Radner
  • Institute University of Graz

Tool Details

  • Tool name: MAGL Knockout mouse
  • Alternate names: MGL, Mgll, Magl
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Knock-Out
  • Conditional: No
  • Genetic background and cross history: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are backcrossed regularly to C57BL/6J to avoid generation of subpopulations.
  • Phenotype: These mice display a mildly attenuated peripheral lipolysis, and are resistant to diet-induced insulin resistance and hepatic steatosis. Further, these mice display a desensitization of intestinal cannabinoid receptor type 1, which leads to accelerated colonic propulsion.
  • Zygosity: Homozygous
  • Strain: C57BL/6J
  • Description: Useful model to study MAGL-deficiency. Animals might be useful for studies, e.g. on energy metabolism or endocannabinoid signalling.
  • Research area: Metabolism; Cell signaling and signal transduction
  • Production details: A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed Mgll exon 3 and 4 and the selection cassette. Further details are available upon request
  • Breeding information: Good breeder. Homozygous mice may be bred together to maintain a live colony.

  • For Research Use Only

Target Details

  • Target: Monoglyceride lipase; monoacyl glycerol lipase

Application Details

Handling

Documentation

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References

  • •  26075589
  • •  Taschler et al. 2011. J Biol Chem. 286(20):17467-77. PMID: 21454566.