EXPERIMENTAL MODELS
Contributor Information
- Name Clive Dickson
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: K5R2b Null Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Cancer type: Digestive / Gastrointestinal cancer
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Fgfr2bflox/flox mouse crossed with K5-Cre mouse (tissue-specific Cre expression in skin) giving tissue-specific knockout of FGFR2 IIIb isoform in skin.
- Application: The K5-R2b-null mouse is an ideal tool in the study of the tumour suppressive role of the FGFR2 IIIb isoform in skin tumourigenesis.Skin tumours develop in K5-R2b-null mice following two-step carcinogen treatment with DMBA and TPA.
- Genetic background and cross history: Homozygous Fgfr2bflox/flox mice, carrying a loxP flanked IIIb exon of FGFR2, were crossed with male heterozygous K5-Cre mice, carrying Cre recombinase transgene under control of the Keratin-5 promoter. Mice were maintained on a mixed 129/C57BL6 background.
- Phenotype: Skin abnormalities, Tumourigenesis
- Zygosity:
- Strain:
- Description: A mouse model with epidermal FgfR 2b expression knocked out. The mouse lives a normal life span but has altered hair morphology. It has a tendency to develop skin papillomas spontaneously and development of these can be accelerated greatly by use of a carcinogen.
- Research area: Cancer; Cell signaling and signal transduction; Genetics; Immunology
- Production details: Homozygous Fgfr2bflox/flox mice, carrying a loxP flanked IIIb exon of FGFR2, were crossed with male heterozygous K5-Cre mice, carrying Cre recombinase transgene under control of the Keratin-5 promoter. Mice were maintained on a mixed 129/C57BL6 background.
- Additional notes: The K5-R2b-null mouse is an ideal tool in the study of the tumour suppressive role of the FGFR2 IIIb isoform in skin tumourigenesis.Skin tumours develop in K5-R2b-null mice following two-step carcinogen treatment with DMBA and TPA.
- For Research Use Only
Target Details
- Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)
Application Details
- Application: The K5-R2b-null mouse is an ideal tool in the study of the tumour suppressive role of the FGFR2 IIIb isoform in skin tumourigenesis.Skin tumours develop in K5-R2b-null mice following two-step carcinogen treatment with DMBA and TPA.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
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