EXPERIMENTAL MODELS
Contributor Information
- Name Ralf H. Adams
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Pdgfrb-Cre Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Cancer; Angiogenesis
- Model: Conditional KO
- Conditional: Yes
- Conditional description: Pdgfrb-Cre enables conditional, pericyte and smooth vascular muscle cell knockout/knockin/transgene expression of loxP flanked genes.
- Genetic background and cross history: A Pdgfrb-Cre transgene vector, containing a genomic Pdgfrb promoter fragment fused to Cre recombinase and a polyA sequence, was injected into fertilised 129/C57BL6 oocytes. Founder lines were backcrossed to establish transgenic lines on a mixed 129/C57BL6 background.
- Description: Cre expressed under Pdgfrb promoter enabling conditional recombine expression in pericytes and smooth vascular muscle cells (tissue-specific loxP knockout/knockin)
- Research area: Cancer; Tissue-specific biology; Genetics; Immunology
- Production details: A Pdgfrb-Cre transgene vector, containing a genomic Pdgfrb promoter fragment fused to Cre recombinase and a polyA sequence, was injected into fertilised 129/C57BL6 oocytes. Founder lines were backcrossed to establish transgenic lines on a mixed 129/C57BL6 background.
- For Research Use Only
Target Details
- Target: Cre recombinase under Pdgfrb promoter
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
Related Tools
References
- • Kogata et al. 2009. Genes Dev. 23(19):2278-83. PMID: 19797768.
- • Integrin-linked kinase controls vascular wall formation by negatively regulating Rho/ROCK-mediated vascular smooth muscle cell contraction.
- • Foo et al. 2006. Cell. 124(1):161-73. PMID: 16413489.
- • Ephrin-B2 controls cell motility and adhesion during blood-vessel-wall assembly.
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