Product Image

Contributor Information

  • Name Riccardo Dalla-Favera
  • Institute The Trustees of Columbia University in the City of New York

Tool Details

  • Tool name: MDR KO Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Chronic Lymphoblastic Leukemia
  • Model: Knock-Out
  • Model description: Knockout mouse of the MDR chromosomal region in m14qC3/h13q14, which encodes the DLEU2 gene and the miR15a/16-1 microRNA cluster
  • Conditional: No
  • Genetic background and cross history: A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Description: Deletion of human chromosomal region 13q14 (mouse 14qC3) represents the most common genetic aberration in B-cell chronic lymphocytic leukaemia (CLL), a neoplasm of mature B lymphocytes. 13q14 deletions are commonly large and heterogeneous in size and affect multiple genes. Contained within the 13q14 region is the 0.11 megabase-long MDR, which encompasses the DLEU2 gene and miR-15a/16-1 cluster. Deletion of the MDR in this model organism recapitulates the full spectrum of CLL-associated lymphoproliferations in humans.
  • Research area: Cancer; Immunology
  • Production details: A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele
  • Additional notes: Knockout mouse of the MDR chromosomal region in m14qC3/h13q14, which encodes the DLEU2 gene and the miR15a/16-1 microRNA cluster

  • For Research Use Only

Target Details

  • Target: Minimal deleted region of h13q14/m14qC3

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

Related Tools

Product Image

Fzd96b_e1[52bp del] Zebrafish

#158357
Product Image

p110beta PI3K-floxed

#158394
Product Image

HBNb Mouse

#151771

References

  • •  Klein et al. 2010. Cancer Cell. 17(1):28-40. PMID: 20060366.
  • •  The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.