EXPERIMENTAL MODELS
Contributor Information
- Name Catrin Pritchard
- Institute University of Leicester
Tool Details
- Tool name: Raf KO Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Cancer; Apoptosis
- Model: Knock-Out
- Conditional: No
- Cell signalling pathway: Ras/Raf signalling
- Genetic background and cross history: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
- Phenotype: Homozygous Raf-1-/- mice die in embryogenesis.
- Zygosity: Heterozygous
- Description: The Raf-/- embryo phenotype shows defects in vascularisation and placental development as well as increased apoptosis of many tissues.
- Research area: Cancer; Cell signaling and signal transduction; Genetics; Neurobiology
- Production details: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
- For Research Use Only
Target Details
- Target: Raf1+/- heterozygous knockout
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
Related Tools
References
- • H
ser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227. - • MEK kinase activity is not necessary for Raf-1 function.
.svg)
Availability: 
.svg)
.svg)
