EXPERIMENTAL MODELS
Contributor Information
- Name Bart Vanhaesebroeck
- Institute Ludwig Institute for Cancer Research
Tool Details
- Tool name: Pik3ca-H1047R conditional Flp inducible
- Tool type: Experimental models
- Tool sub-type: Mouse
- Model: Conditional KO
- Conditional: Yes
- Cell signalling pathway: PI3K/AKT signalling
- Genetic background and cross history: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110akinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
- Phenotype: Homozygous and heterozygous mice are phenotypically normal.
- Research area: Cancer
- Production details: Genomic DNA covering the last 6 exons of the coding region of Pik3ca was isolated and a point mutation, resulting in conversion of CAT (H) to CGT (R) in the C terminal p110Îąkinase domain, was introduced by site-directed mutagenesis. In addition, a neo selection cassette flanked by frt sites was inserted into exons 19 and 20 in the targeting vector using standard restriction enzyme- and PCR-based cloning techniques.
- Additional notes: Homozygous and heterozygous mice are phenotypically normal.
- For Research Use Only
Target Details
- Target: PIK3CA
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
Related Tools
References
- • Berenjeno et al. 2017. Nat Commun. 8(1):1773. PMID: 29170395.
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