EXPERIMENTAL MODELS
Contributor Information
- Name Eddie Wang ; Mike Owen
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
- Primary citation Wang et al. 2001. Mol Cell Biol. 21(10):3451-61. PMID: 11313471
Tool Details
- Tool name: DR3 KO Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Progressive neurological disease
- Model: Knock-Out
- Conditional: No
- Application: In vivo studies of DR3-induced apoptosis & function; in vivo studies of negative T cell selection and development
- Phenotype: Immune abnormalities (T cell development);
- Zygosity: Homozygous
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Description: The DR3-/- mouse exhibits complete knockout of DR3, a death domain-containing tumour necrosis factor receptor, which mediates one of the key regulators of the cell division cycle. Negative selection and anti-CD3-induced apoptosis are significantly impaired in DR3-null mice. In contrast, both superantigen-induced negative selection and positive selection are normal. The pre-T-cell receptor-mediated checkpoint, which is dependent on TNFR signaling, is also unaffected in DR3-deficient mice. These data reveal a nonredundant in vivo role for this TNF receptor family member in the removal of self-reactive T cells in the thymus.
Other behavioral studies on DR3 KO mice indicated that DR3 plays a key nonredundant role in the retention of normal motor control function during aging in mice and implicate DR3 in progressive neurological disease - Research area: Apoptosis and autophagy; Cancer; Cell biology; Cell signaling and signal transduction; Genetics; Immunology
- Production details: A DR3 targeting vector, replacing the entire DR3 coding region with a loxP flanked resistance cassette, was transfected into GK129 ES cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
- For Research Use Only
Target Details
- Target: DR3 (Death Domain Receptor 3)
- Target background: DR3 (Ws1, Apo3, LARD, TRAMP, TNFSFR12) is a member of the death domain-containing tumor necrosis factor receptor (TNFR) superfamily, members of which mediate a variety of developmental events including the regulation of cell proliferation, differentiation, and apoptosis
Application Details
- Application: In vivo studies of DR3-induced apoptosis & function; in vivo studies of negative T cell selection and development
Handling
- Format: Embryo/Spermatoza
- Shipping conditions: Dry Ice
Documentation
Related Tools
References
- • Twohig et al. 2010. J Neurosci. 30(10):3782-92. PMID: 20220013
- • Wang et al. 2001. Mol Cell Biol. 21(10):3451-61. PMID: 11313471
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