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Contributor Information

  • Name Alessandra Recchia
  • Institute Universit̕ di Modena e Reggio Emilia

Tool Details

  • Tool name: P23H RHO HeLa cell line
  • Alternate names: RHO
  • Tool type: Cell Lines
  • Parental cell line: HeLa cell line
  • Organism: Human
  • Tissue: Cervix
  • Cancer type: Eye cancer
  • Disease: Cancer
  • Model: Immortalised Line
  • Conditional: No
  • Application: Rhodopsin
  • Description: The HeLa cell lines stably expressing RHO gene carrying the P23H dominant mutation (P23H-RHO) were generated because no cell line expressing RHO was available. Thus, mRNA and protein analyses can be assessed in the P23H RHO HeLa cell line after genome editing.
  • Research area: Genetics
  • Production details: The cell model was produced by transducing HeLa cells with a lentiviral vector coding for P23H RHO variant under the phosphoglycerate kinase (PGK) promoter

  • For Research Use Only

Target Details

  • Target: Rhodopsin

Application Details

  • Application: Rhodopsin

Handling

  • Format: Frozen
  • Growth medium: The cell model was produced by transducing HeLa cells with a lentiviral vector coding for P23H RHO variant under the phosphoglycerate kinase (PGK) promoter Dulbeccoâ?‚€?‚™s modified Eagleâ?‚€?‚™s medium (DMEM) supplemented with 10% foetal calf serum (FCS), 100 U/ml, penicillin and 100 mg/ml streptomycin.

Documentation

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References

  • •  Latella et al. 2016. Mol Ther Nucleic Acids. 5(11):e389. PMID: 27874856.