CELL LINES
Contributor Information
- Name Pranab K Das
Tool Details
- Tool name: Immortalised Human Fibroblast Cell Line
- Tool type: Cell Lines
- Parental cell line: Human foreskin
- Organism: Human
- Tissue: Fibroblast
- Growth properties: Cells were routinely passaged 1:4 at confluency
- Model: Immortalised Line
- Description: Immortalised human fibroblasts are widely employed as a model system for cell and molecular biology studies
- Research area: Cell biology
- Production details: Fibroblast cultures were establised from neonatal foreskin using standard methods. Fibroblasts were transfected with HPV16 genes E6 and E7 using the retroviral construct LXCN16E6E7. The E6E7 genes are under the control of the MMLV promoter-enhancer sequence. In additon the vector contained a geneticin resistance gene. The retroviral particles were produced by the packaging cell line PA317. The critical concentration of geneticin for transformed selection was 1mg/ml.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM:M199 (1:4) supplemented with 10% FBS
- Shipping conditions: Dry ice
Documentation
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