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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society
  • Primary citation Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.

Tool Details

  • Tool name: T47D/S2 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: T47D
  • Organism: Human
  • Tissue: Breast
  • Gender: Female
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Description: The T47D/S2 cell line is a control cell line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 cell lines. This cell line has been established by the reduction of the serum concentration in order to enable growth inhibiton with tamoxifen. T47D/S2 is adherent and the morphology is polygonal epithelial.

    T47D/S2 cells express oestrogen receptor (alpha) and progesterone receptor.

    This cell line allows the study of the mechanisms involved in tamoxifen resistant breast cancer cell growth.
  • Research area: Cancer; Drug development
  • Production details: T47D/S2 cells have been established by reduction of the serum concentration to 2% fetal calf serum, in order to enable growth inhibition with tamoxifen.
  • Cellosaurus ID: CVCL_1D45

  • For Research Use Only

Target Details

  • Target: Oestrogen receptor

Application Details

  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Application notes: T47DS2 cells express ER alpha and progesterone receptor.

Handling

  • Format: Frozen
  • Passage number: Passage 151 (AL3707, AL3708)
  • Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
  • •  Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
  • •  Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
  • •  Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.