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Contributor Information

  • Name Irene Leigh
  • Institute Queen Mary University of London
  • Primary citation Inman et al. 2018. Nat Commun. 9(1):3667. PMID: 30202019

Tool Details

  • Tool name: MET1 SCC Cell Line
  • Alternate names: MET-1; MET1; SCCT; Squamous Cell Carcinoma line; SCC; Keratinocyte
  • Clone: MET1
    The difference between clones: keratinocyte lines MET1, MET2, MET3 and MET4 were derived from the same individual, but from varying stages in progression of an SCC: invasive, recurrent and, subsequently metastatic.
    The clones were derived, respectively, from an SCC excised from the back of the left hand (MET1), from 2 recurrent SCC arising at the same site in rapid succession (MET2 and 3) and from metastatic SCC within left axillary lymph nodes biopsied following the third excision (MET4)
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: A male, 45 y.o. immunosuppressed patient with a primary invasive SCC on the back of the hand that subsequently recurred locally twice. Immune status: renal transplant; Immune therapy: Azathioprine, Prednisolone; Site: dorsum left hand; Histology: MD; Germline DNA: dermal fibroblasts. Same donor: PM1, MET1,2,3,4, T9 cell lines
  • Tissue: Skin
  • Gender: Male
  • Cancer type: Skin cancer; Skin squamous cell carcinoma; Cutaneous squamous cell carcinoma; cSCC
  • Model: Tumour line
  • CRISPR: No
  • Conditional: No
  • Application: Early neoplastic progression studies; Genetic analysis of the malignant process; Research on aggressive primary SCC after it has
  • Description: Keratinocyte cell line representing the primary acantholytic carcinoma, that originated from an invasive and ultimately metastatic SCC.
    MET1 is derived from a clinical progression of a primary epidermal tumour from the back of a left hand through to an invasive and metastatic lesion. Histology of the original tumour (from which MET-1 was derived) showed an acantholytic squamous cell carcinoma.
    MET1 has been used to understand aggressive primary SCC after it has metastasised. It is an important cell line for this specific stage in SCC cancer progression. MET1 is the first cell line in a unique series of epidermal cell lines representing different stages of malignant transformation, that were derived from a single, adult, immunosuppressed individual.
    Four keratinocyte lines, PM1-4, established from forehead skin are here compared with 4 squamous cell carcinoma (SCC) lines, MET1-4, derived respectively from a primary cutaneous tumour, two local recurrences and a distant metastasis of invasive SCC. Despite altered growth properties, the PM lines retained many features of normal keratinocytes including keratin phenotype, differentiation capacity and non-tumorigenicity in athymic mice. In contrast, from early passage, the MET lines displayed markedly reduced growth requirements, abnormal differentiation, aberrant K18 expression and tumorigenicity in athymic mice. The abnormal keratin profile of individual MET lines closely reflected the keratin phenotype of the tumour of origin. Although unusual HPV types were identified in the original tissue, there was no evidence of persistent virus within any cell line and it appears that HPV is not critical for maintenance of the immortal phenotype. The PM lines were distinctly different from invasive SCC lines and are likely to be useful in studies focusing on mutations that are important in early neoplastic progression.
    The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process
  • Research area: Cancer
  • Production details: MET1 was derived from a primary and invasive SCC excised from the back of the left hand according to a previously described method [PMID: 21516406]
  • Cellosaurus ID: CVCL_LN09
  • Additional notes: IC1 and IC1MET SCC Cell Lines cells are the most successful in xenografts and surface xenotransplantation (unpublished)

  • For Research Use Only

Target Details

Application Details

  • Application: Early neoplastic progression studies; Genetic analysis of the malignant process; Research on aggressive primary SCC after it has

Handling

  • Format: Frozen
  • Growth medium: Keratinocyte medium: 3:1 V/V mixture of DMEM and Ham’s F12 supplemented with 10% FBS and a cocktail of mitogens: 0.5 ug/ml hydrocortisone; 1x10-10M cholera toxin; 1.8 x 10-4 M adenine; 2 x 10–11 M liothyronine, 13 ng/ml; 5 μg/mL insulin; 10 ng/mL epidermal growth factor (EGF). FBS (10%) is routinely batch tested for enabling optimal clonal growth of cells. Culture SCC cell lines in the presence of mitotically inactivated 3T3-J2 feeder layers, which enhance proliferative capacity and reduces selective pressure on the cancer cells.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Characterisation tests: Genetic analysis [PMID: 30202019]
  • Mycoplasma free: Yes
  • Biosafety level: 1
  • Subculture routine: Passage upon attaining 80% confluence

Documentation

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References

  • •  Hassan et.al. 2019. Int J Mol Sci. 20(14):3428. PMID: 31336867
  • •  Proby et al. 2000. Exp Dermatol. 9(2):104-17. PMID: 10772384
  • •  Popp et.al. 2000. J Invest Dermatol. 115(6):1095-103. PMID: 11121147
  • •  Abikhair Burgo et al. 2018. JCI Insight. 3(17):. PMID: 30185657
  • •  McHugh et.al. 2018. Oncotarget 9(29):20265-20281. PMID: 29755650
  • •  Inman et al. 2018. Nat Commun. 9(1):3667. PMID: 30202019