CELL LINES
Contributor Information
- Name Akira Ono
- Institute University of Michigan
Tool Details
- Tool name: Immortalized Fibroblastic Reticular cell line
- Tool type: Cell Lines
- Parental cell line: Human lymphatic fibroblasts
- Organism: Human
- Tissue: Lymph Node
- Model: Immortalised Line
- Description: Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection.
- Research area: Immunology
- Production details: Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: The cell line was maintained in fibroblast medium containing 2% FBS, P/S, and fibroblast growth supplement and subsequently selected with 1000ÄË?Â?ÂÄÂ??g/ml G418.
- Shipping conditions: Dry ice
Documentation
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