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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society, Denmark
  • Primary citation Hole et al. 2015. Int J Oncol. 46(4):1481-1490. PMID: 25625755.

Tool Details

  • Tool name: MCF7/ExeR-1 Cell Line
  • Alternate names: MCF-7/ExeR-1; ExeR-1
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7
  • Organism: Human
  • Tissue: Breast
  • Gender: Female
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Growth properties: Breast cancer cell line resistant to the aromatase inhibitor exemestane. Estrogen receptor positive. Progesterone receptor positive when grown in medium without exemestane.
  • Conditional: No
  • Application: Investigation of molecular mechanisms of exemestane resistance
  • Description: MCF7/ExeR-1 is a cell culture model mimicking acquired resistance of aromatase inhibitors (AIs) - an anti-cancer therapy. This breast cancer cell line was established from MCF7 cells. The cellular classification is epithelial, and their shape is polygonal.

    The MCF7/ExeR-1 cell line is resistant to the third generation AI - exemestane (Aromasin). Third generation AIs have proven to be effective treatment for oestrogen receptor positive (ER+) breast cancer and as such are recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients. However, a major problem is development of resistance against AIs.

    Previous applications of this cell line include western blot analysis of protein expression.

    Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the non-steroidal AI exemestane allows the study of the molecular basis for AI resistance to find new targets for treatment.
  • Research area: Cancer; Drug development
  • Production details: Exemestane-resistant cell lines were established from MCF-7 cells grown in medium with 10% NCS and 10-7 M testosterone. A culture of MCF-7 cells were treated with 10-7 M exemestane for one week, trypsinised and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with exemestane. After ~2-3 months, the isolated colonies gave rise to exemestane-resistant cell lines, which could be grown in exemestane-containing medium with a weekly split ratio of ~1:25. The MCF-7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF-7 cell line.
  • Cellosaurus ID: CVCL_5A13

  • For Research Use Only

Target Details

  • Target: Exemestane resistance

Application Details

  • Application: Investigation of molecular mechanisms of exemestane resistance
  • Application notes: Human breast cancer cell line derived from MCF-7 cells Other related cell lines: - LetR-1, LetR-2, LetR-3 and LetR-4 resistant to the non-steroidal AI letrozole - ExeR-2, ExeR-3 and ExeR-4 resistant to the steroidal AI exemestane - AnaR-1, AnaR-2, AnaR-3 and AnaR-4 resistant to the non-steroidal AI anastrozole Passage 430 (AL3058, AL3059)

Handling

  • Format: Frozen
  • Passage number: Passage 430 (AL3058, AL3059)
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM exemestane.
  • Temperature: 37° C
  • Atmosphere: Humidified air with 5% CO2
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-726. PMID: 25667100.
  • •  Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.