CELL LINES
Contributor Information
- Name Amy Chadwick
- Institute University of Liverpool, Molecular and Clinical Pharmacology
Tool Details
- Tool name: UOL-Hep-CYB6 cell line
- Tool type: Cell Lines
- Parental cell line: HepG2
- Organism: Human
- Tissue: Liver
- Disease: Mitochondrial Subhaplogroup : H1a1
- Growth properties: Adherent
- Model description: Cybrid Cell Line
- Description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
- Research area: Drug development
- Additional notes: Parental cell line was purchased from ECACC
- For Research Use Only
Target Details
Application Details
- Application notes: SNPs characteristic of assigned haplogroup : 73G 263G 750G 1438G 3010A 4769G 6365C 8860G 15326G 16162G Additional SNPs: 152C 3483A 16360T 16519C
Handling
- Growth medium: DMEM high-glucose medium (glucose; 25?mM) supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), and HEPES (1?mM)
- Temperature: 37C
- Atmosphere: 5% CO2
- Subculture routine: Cells are subcultured weekly (1:12) via trypsinisation.
Documentation
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