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Contributor Information

  • Name Peter Nghiem
  • Institute Texas A&M University

Tool Details

  • Tool name: Myok9 cell line
  • Tool type: Cell Lines
  • Organism: Canine
  • Tissue: Skeletal
  • Disease: Muscle Disorders; Duschenne Muscular dystrophy (DMD); Myotubular myopathy; Canine myopathies
  • Model: Immortalised Line
  • Conditional: No
  • Description: This Myok9 cell line is derived from skeletal muscle from a genotypically normal dog and has been immortalized to study muscle disorders in canines. This cell line expresses dystrophin and other muscle-specific proteins during differentiation.
  • Production details: Surgical biopsy of vastus lateralis muscle was performed on a 3-year old normal golden retriever. Myoblast extraction using pre-plate technique was performed. Primary cells were extracted and immunostained for desmin and PAX7. Immortalization was performed with lentivirus encoding SV40 large T antigen with puromycin resistance gene at MOI 2. Cells were selected with 1ug/ml puromycin for 3 days and passaged for 3 cycles.

  • For Research Use Only

Target Details

  • Target: canine muscle cell line

Application Details

Handling

  • Format: Frozen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  L?„‚Ě‚pez et al. 2020. Mamm Genome. 31(3-4):95-109. PMID: 32246189.