ANTIBODIES
Contributor Information
- Name Daniel Geraghty
- Institute Fred Hutchinson Cancer Research Center
- Primary citation lshitani et al.2003. Journal of lmmunology. 171(3):1376-84. PMID: 12874228
Tool Details
- Tool name: Anti-HLA-G [87G]
- Clone: 87G
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Cancer type: Skin cancer; Breast cancer; Genitourinary cancer; Blood cancer
- Molecular weight of the target: The heavy chain is approximately 45 kDa
- Application: FACS, IP(weak), ELISA, Histology. No western
- Description: HLA-G has long been assumed a major factor in this immune accommodation because of its localized expression and because of low levels of allelic polymorphism. This MoAb was developed to examine the role of all three nonclassical class I Ags in the maternal immune response during pregnancy. MoAb 87G recognizes recognizes human HLA-G, a member of the Human Leukocyte Antigen family but as part of the nonclassical MHC type involved in inhibiting immune reponses.
- Isotype: IgG2a,?
- Research area: Immunology; Developmental biology
- For Research Use Only
Target Details
- Target: HLA-G
- Target molecular weight: The heavy chain is approximately 45 kDa
- Target background: HLA-G are expressed in the placenta, a membrane form and a soluble form, the latter containing an altered carboxyl terminu. HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (?2 microglobulin). The heavy chain is anchored in the membrane.
Application Details
- Application: FACS, IP(weak), ELISA, Histology. No western
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage buffer: PBS, pH 7.2
- Storage conditions: 4° C
Documentation
References
- • lshitani et al.2003.Journal of lmmunology. 171(3):1376-84.PMID: 12874228
- • Lee et al.1995. Immunity.(5):591-600.PMID: 7584149
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