ANTIBODIES

Contributor Information
- Name Daniel Geraghty
- Institute Fred Hutchinson Cancer Research Center
- Primary citation lshitani et al. 2003. Journal of Immunology. 171(3):1376-84. PMID: 12874228
Tool Details
- Tool name: Anti-HLA-E [3D12]
- Clone: 3D12
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: ~40kDa
- Application: FACS, IP, ELISA, Histology, No Western Blot.
- Description: MoAb was combined to examine HLA expression in placental tissues for all the known forms of the nonclassical HLA-E class I molecules that is important in the maternal immune response during pregnancy. Antibody 3D12 showed that HLA-E peptide binding was correlated with surface expression.
- Isotype: IgG1
- Research area: Immunology; Developmental biology
- For Research Use Only
Target Details
- Target: HLA-E class I complexes
- Target molecular weight: ~40kDa
- Target background: Antibody is used to examine expression in placental tissues for all the known forms of the HLA-E nonclassical class I molecules.
Application Details
- Application: FACS, IP, ELISA, Histology, No Western Blot.
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
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References
- • lshitani et al. 2003. Journal of Immunology. 171(3):1376-84.PMID: 12874228
- • Lee et al. 1998. J. Immunol.?160(10):4951-60. PMID: 9590243