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Contributor Information

  • Institute The Institute of Cancer Research

Tool Details

  • Tool name: Anti-Rheb [Rheb 3H6]
  • Clone: Rheb 3H6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IP ; WB
  • Description: Monoclonal antibody which binds the Rheb GTP binding protein, and can be used to help understand the role of Rheb in mTORC signalling.
  • Immunogen: Human Rheb GST fusion
  • Immunogen UniProt ID: Q15382
  • Isotype: IgG1
  • Research area: Cancer; Cell signaling and signal transduction; Metabolism

  • For Research Use Only

Target Details

  • Target: Rheb
  • Target background: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras superfamily of small GTP binding proteins. Rheb is expressed at high levels in the brain, but is also found in many other tissues, and can be induced by growth factor stimulation. It is ubiquitously expressed in humans and other mammals. Rheb triggers activation of the Raf-MEK-MAPK pathway and has an important role in regulating the insulin/Target of rapamycin (TOR) signalling pathway, and the cell cycle. Rheb stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signalling. Overexpression of Rheb can be seen in multiple human carcinomas.

Application Details

  • Application: IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Findlay et al. 2007. Biochem J. 403(1):13-20. PMID: 17253963.
  • •  A MAP4 kinase related to Ste20 is a nutrient-sensitive regulator of mTOR signalling.