ANTIBODIES

Contributor Information
- Name Walter Bodmer
- Institute University of Oxford
Tool Details
- Tool name: Anti-CDX1 [123a]
- Alternate names: Caudal Type Homeobox 1
- Clone: 123a
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 32 kDa
- Application: ChIP ; IF ; WB ; ChIP-seq
- Strain: Balb/c
- Description: CDX1 is an intestine-specific transcription factor with a role in directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. Cdx1 positively regulates its own expression in the small intestine and colon of fetus and adult and promotes cellular growth and differentiation in epithelial intestinal cells. A reduction in human Cdx1 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.
- Immunogen: Human CDX-1 N-terminal peptide
- Isotype: IgG1
- Research area: Apoptosis and autophagy; Cancer; Genetics
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: CDX1
- Target molecular weight: 32 kDa
- Target background: CDX1 is an intestine-specific transcription factor with a role in directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. Cdx1 positively regulates its own expression in the small intestine and colon of fetus and adult and promotes cellular growth and differentiation in epithelial intestinal cells. A reduction in human Cdx1 expression is associated with colorectal tumorigenesis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.
Application Details
- Application: ChIP ; IF ; WB ; ChIP-seq
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
Related Tools
References
- • Jones et al. 2015. Proc Natl Acad Sci U S A. 112(13):E1550-8. PMID: 25775580.
- • The CDX1-microRNA-215 axis regulates colorectal cancer stem cell differentiation.
- • Ashley et al. 2013. Cancer Res. 73(18):5798-809. PMID: 23867471.
- • Stem cell differentiation and lumen formation in colorectal cancer cell lines and primary tumors.
- • Yeung et al. 2011. Proc Natl Acad Sci U S A. 108(11):4382-7. PMID: 21368208.
- • Hypoxia and lineage specification of cell line-derived colorectal cancer stem cells.
- • Yeung et al. 2010. Proc Natl Acad Sci U S A. 107(8):3722-7. PMID: 20133591.
- • Cancer stem cells from colorectal cancer-derived cell lines.
- • Chan et al. 2009. Proc Natl Acad Sci U S A. 106(6):1936-41. PMID: 19188603.
- • Gastrointestinal differentiation marker Cytokeratin 20 is regulated by homeobox gene CDX1.