ANTIBODIES
Contributor Information
- Name Richard Nash
- Institute Fred Hutchinson Cancer Research Center
- Primary citation McSweeney et al. 1998.Blood. 91(6):1977-86. PMID: 9490680
Tool Details
- Tool name: Anti-CD34 [2E9]
- Clone: 2 e9
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Canine
- Molecular weight of the target: 110kDa
- Application: Western Blotting, Flow Cytometry, CFU-GM assay, LTC-IC assay, ELISA
- Description: Good animal models in which to refin studies of CD34 cell fractions are limited. However, this MoAb detected 4% to 17% CD34 cells in murine Bone Marrow (BM) cells, somewhat higher than the 1% to 5% CD34 cells detected in human BM. Can be used to enrich canine progenitor cell populations that appear functionally and phenotypically similar to human CD34 cell
- Immunogen: recombinant CD34 murine Ig fusion molecule (CD34-Ig)
- Isotype: IgG-1
- Research area: Stem cell biology
- For Research Use Only
Target Details
- Target: CD34
- Target molecular weight: 110kDa
- Target background: CD34 has previously been define as a marker of hematopoietic progenitors and is expressed on canine bone marrow (BM) progenitor cells in a manner analogous to that found in humans.
Application Details
- Application: Western Blotting, Flow Cytometry, CFU-GM assay, LTC-IC assay, ELISA
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.
Documentation
Related Tools
References
- • McSweeney et al. 1998.Blood. 91(6):1977-86.PMID:PMID: 9490680
- • Wayner et al. 1987.J Cell Biol.105 (1987): 1873.https://doi.org/10.1083/jcb.105.4.1873
.svg)
.svg)
