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Contributor Information

  • Name Barry McDonnell ; Caroline Murphy
  • Institute Dublin City University

Tool Details

  • Tool name: Anti-Myeloperoxidase (MPO) scFv (B10B) [B10BscFv]
  • Alternate names: MPO
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Recombinant
  • Conjugate: Unconjugated
  • Host: Chicken
  • Application: ELISA ; WB
  • Description: MPO is a mediator enzyme secreted by inflammatory cells e.g. activated neutrophils and monocytes. MPO produces hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion (Cl-) (or the equivalent from a non-chlorine halide) during the neutrophil's respiratory burst, requiring heme as a cofactor. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent (Heinecke et al., 1993). Both hypochlorous acid and tyrosyl radical are cytotoxic and are used by neutrophils to kill bacteria and other pathogens. High levels of MPO have been reported in atherosclerotic plaques (Blake and Ridker, 2003) and accumulating evidence indicates that MPO may also have a causative role in plaque vulnerability (Hazen, 2004). MPO can generate several reactive oxidised intermediates, concomitantly inducing oxidative damage of cells and tissues (Brennan et al., 2003).
  • Immunogen: Myeloperoxidase
  • Immunogen UniProt ID: TBC
  • Research area: Tissue-specific biology

  • For Research Use Only

Target Details

  • Target: Myeloperoxidase
  • Target background: MPO is a mediator enzyme secreted by inflammatory cells e.g. activated neutrophils and monocytes. MPO produces hypochlorous acid (HOCl) from hydrogen peroxide (H2O2) and chloride anion (Cl-) (or the equivalent from a non-chlorine halide) during the neutrophil's respiratory burst, requiring heme as a cofactor. Furthermore, it oxidizes tyrosine to tyrosyl radical using hydrogen peroxide as an oxidizing agent (Heinecke et al., 1993). Both hypochlorous acid and tyrosyl radical are cytotoxic and are used by neutrophils to kill bacteria and other pathogens. High levels of MPO have been reported in atherosclerotic plaques (Blake and Ridker, 2003) and accumulating evidence indicates that MPO may also have a causative role in plaque vulnerability (Hazen, 2004). MPO can generate several reactive oxidised intermediates, concomitantly inducing oxidative damage of cells and tissues (Brennan et al., 2003).

Application Details

  • Application: ELISA ; WB

Handling

  • Format: Liquid
  • Storage buffer: 1 x PBS
  • Storage conditions: -20°C, avoid repeated freeze and thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  McDonnell, Barry (2010) Development of novel antibody-based diagnostics for the early and rapid detection of cardiac markers. PhD thesis, Dublin City University.