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Contributor Information

  • Name Jo Morris ; Ruth Densham
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-pS114-BRCA1 [3C10G8]
  • Alternate names: pS114, BRCA1
  • Clone: BRCA1(pS114) antibody
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Application: IF ; IP ; WB
  • Description: BRCA1 proteins protect stalled replication forks from degradation by nucleases, through pathways that involve RAD51. Specifically, BRCA1 in complex with BARD1, and not the canonical BRCA1-PALB2 interaction, is required for fork protection. BRCA1-BARD1 is regulated by a conformational change mediated by the phosphorylation-directed prolyl isomerase PIN1. PIN1 activity enhances BRCA1-BARD1 interaction with RAD51, thereby increasing the presence of RAD51 at stalled replication structures.
  • Research area: Genetics

  • For Research Use Only

Target Details

  • Target: pS114-BRCA1
  • Target background: BRCA1 proteins protect stalled replication forks from degradation by nucleases, through pathways that involve RAD51. Specifically, BRCA1 in complex with BARD1, and not the canonical BRCA1PALB2 interaction, is required for fork protection. BRCA1BARD1 is regulated by a conformational change mediated by the phosphorylation-directed prolyl isomerase PIN1. PIN1 activity enhances BRCA1BARD1 interaction with RAD51, thereby increasing the presence of RAD51 at stalled replication structures.

Application Details

  • Application: IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Daza-Martin et al. 2019. Nature. 571(7766):521-527. PMID: 31270457.