Product Image

Contributor Information

  • Name Walter Bodmer
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-APC [c-APC 28.9]
  • Clone: c-APC 28.9
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Digestive / Gastrointestinal cancer
  • Molecular weight of the target: 320 kDa
  • Application: IHC ; IF ; IP ; WB
  • Description: C-APC 28.8 can be used for APC expression and detection of APC mutations.
  • Immunogen: human APC C-terminal fusion with MBP (maltose binding protein)
  • Isotype: IgG1 kappa
  • Research area: Cancer; Tissue-specific biology; Cell biology; Cell signaling and signal transduction; Genetics; Neurobiology; Stem cell biology

  • For Research Use Only

Target Details

  • Target: APC
  • Target molecular weight: 320 kDa
  • Target background: The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Clone c-APC 28.9 is useful for the detection of APC expression and detection of mutations. This antibody has been used routinely for Western blotting on colon cell line HCT116 extract which expresses full-length APC.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Kariola et al. 2005. Fam Cancer. 4(2):187-90. PMID: 15951972.
  •   APC and beta-catenin protein expression patterns in HNPCC-related endometrial and colorectal cancers.
  •   Fodde et al. 2001. Nat Cell Biol. 3(4):433-8. PMID: 11283620.
  •   Mutations in the APC tumour suppressor gene cause chromosomal instability.