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Contributor Information

  • Name Dawen Cai
  • Institute University of Michigan

Tool Details

  • Tool name: "Brainbow"
  • Tool type: Antibodies
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Application: IF ; IP ; WB
  • Description: The "Brainbow" polyclonal antibody has been used in a multicolour labelling strategy for the fluorescent imaging of neuronal circuits and individual neurons in mice, Drosophila and Zebrafish and non-neuronal cells in mice. The “Brainbow” toolkit allows scientists to image highly complex tissue structures by relying on a stochastic method for making different expression ratio combinations of fluorescent proteins so that structures in proximity can be resolved. The antibodies recognize specific fluorescent proteins including EGFP, mCherry, mTFP, TagRFP, PhiYFP, mKusabira-Orange2,TagYFP and mNeonGreen and were produced in various host species: chicken, rabbit, rat and guinea pig.
  • Research area: Neurobiology

  • For Research Use Only

Target Details

  • Target: EGFP, mCherry, mTFP, TagRFP, PhiYFP, mKusabira-Orange2,TagYFP and mNeonGreen
  • Target background: The "Brainbow" polyclonal antibody has been used in a multicolour labelling strategy for the fluorescent imaging of neuronal circuits and individual neurons in mice, Drosophila and Zebrafish and non-neuronal cells in mice. The “Brainbow” toolkit allows scientists to image highly complex tissue structures by relying on a stochastic method for making different expression ratio combinations of fluorescent proteins so that structures in proximity can be resolved. The antibodies recognize specific fluorescent proteins including EGFP, mCherry, mTFP, TagRFP, PhiYFP, mKusabira-Orange2,TagYFP and mNeonGreen and were produced in various host species: chicken, rabbit, rat and guinea pig.

Application Details

  • Application: IF ; IP ; WB

Handling

  • Format: Liquid
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Roossien et al. 2019. Bioinformatics. 35(18):3544-3546. PMID: 30715234.
  • •  Chen et al. 2018. Mol Psychiatry. 23(7):1614-1625. PMID: 28761082.
  • •  Takesian et al. 2018. Nat Neurosci. 21(2):218-227. PMID: 29358666.
  • •  Roossien et al. 2017. Methods Mol Biol. 1642:211-228. PMID: 28815503.
  • •  Manent et al. 2017. Oncogene. 36(40):5576-5592. PMID: 28581519.
  • •  Chang et al. 2017. Nat Methods. 14(6):593-599. PMID: 28417997.
  • •  Zanca et al. 2017. Genes Dev. 31(12):1212-1227. PMID: 28724615.
  • •  Tillberg et al. 2016. Nat Biotechnol. 34(9):987-92. PMID: 27376584.
  • •  Cai et al. 2013. Nat Methods. 10(6):540-7. PMID: 23817127.