ANTIBODIES

Contributor Information
- Institute Yale University
Tool Details
- Tool name: Anti-CD45 [C363.16A]
- Alternate names: YCD45R-1
- Tool type: Antibodies
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Mouse
- Host: Rat
- Molecular weight of the target: 190 kDa and 220 kDa
- Application: FACS ; IF ; IP ; WB
- Description: Monoclonal antibody directed against CD45RB isoform of CD45. Background and Research Application CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, na?ve T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a critical role in TCR and BCR signalling. As T cells become activated and progress from na?ve to memory cells, CD45RB expression is downregulated. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signalling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis.
- Immunogen: cloned T cell lines belonging to the helper T cell (Th2) subset, final boost was given with helper/Th2 clone D10
- Immunogen UniProt ID: P08575
- Isotype: IgG
- Research area: Immunology
- For Research Use Only
Target Details
- Target: CD45
- Target molecular weight: 190 kDa and 220 kDa
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Target background: Monoclonal antibody directed against CD45RB isoform of CD45.
Background and Research Application
CD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, na
ve T cells, thymocytes, weakly on macrophages, and dendritic cells. CD45 regulates Src-family kinases, integrin-mediated signal transduction pathways and can suppress JAK kinases, acting as a negative regulator of cytokine receptor signalling. It plays a critical role in TCR and BCR signalling. As T cells become activated and progress from na ve to memory cells, CD45RB expression is downregulated. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signalling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis.
Application Details
- Application: FACS ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20ðC frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4ðC
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References
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- • Imbratta et al. 2019. Sci Rep. 9(1):6135. PMID: 30992496.
- • Lyons et al. 2018. PLoS Biol. 16(3):e2002417. PMID: 29596476.
- • Lyons et al. 2018. Sci Signal. 11(519):. PMID: 29487189.
- • Bottomly et al. 1989. Eur J Immunol. 19(4):617-23. PMID: 2567241.