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Anti-MBP [BBMBP23.42]

Cat. #154470

Tool name: Anti-MBP [BBMBP23.42]
Tool type: Antibodies
Tool sub-type: Primary Antibody
Class: Monoclonal
Conjugate: Unconjugated
Reactivity: E.coli
Host: Mouse
Molecular weight of the target: MBP precursor polypeptide 43 kDa, mature MBP 42 kDa
Application: IF ; WB
Description: The E. coli maltose binding protein (MBP) is a product of the malE gene and is part of two multicomponent systems that uptake and sense maltose and higher-order glucose polymers (e.g. maltodextrin). MBP acts in the periplasmic space where it binds with high affinity to maltose/maltodextrin which, in turn binds to (i) a transport apparatus that translocates the MBP ligand across the inner membrane to the cytoplasm and (ii) chemoreceptors causing activation of a signaling pathway that directs taxis toward maltose (1). MBP is widely used as an affinity tag for purification of recombinant proteins. The reversible and high-affinity binding of MBP to amylose resins enables recovery of recombinant proteins at high purity in a single purification step. The purification and elution are performed under mild conditions, preserving the activity of the recombinant protein. In addition, MBP might increase expression, proper folding, and solubility, of its fusion partner (2ÄËĂÂĂÂ4).
Immunogen: E.coli maltose binding protein (MBP) fusion construct
Immunogen UniProt ID: P0AEX9
Isotype: IgG2a kappa
Research area: Genetics

Target: Maltose Binding Protein
Target molecular weight: MBP precursor polypeptide 43 kDa, mature MBP 42 kDa
Target background: The E. coli maltose binding protein (MBP) is a product of the malE gene and is part of two multicomponent systems that uptake and sense maltose and higher-order glucose polymers (e.g. maltodextrin). MBP acts in the periplasmic space where it binds with high affinity to maltose/maltodextrin which, in turn binds to (i) a transport apparatus that translocates the MBP ligand across the inner membrane to the cytoplasm and (ii) chemoreceptors causing activation of a signaling pathway that directs taxis toward maltose (1). MBP is widely used as an affinity tag for purification of recombinant proteins. The reversible and high-affinity binding of MBP to amylose resins enables recovery of recombinant proteins at high purity in a single purification step. The purification and elution are performed under mild conditions, preserving the activity of the recombinant protein. In addition, MBP might increase expression, proper folding, and solubility, of its fusion partner (24).

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