ANTIBODIES
Contributor Information
- Name Peter Stern
- Institute Cancer Research UK, Manchester Institute
Tool Details
- Tool name: Anti-5T4 [B3F1]
- Alternate names: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
- Clone: B3F1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Mouse
- Host: Mouse
- Molecular weight of the target: 46.5 kDa
- Application: ELISA ; FACS ; IF ; Fn ; WB
- Strain: C57BL/6
- Description: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.
- Immunogen: Q9Z0L0
- Immunogen UniProt ID: Q9Z0L0
- Isotype: IgG2a
- Research area: Cancer; Stem cell biology
- For Research Use Only
Target Details
- Target: 5T4 oncofetal glycoprotein
- Target molecular weight: 46.5 kDa
- Target background: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.
Application Details
- Application: ELISA ; FACS ; IF ; Fn ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20Ă°C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4Ă°C
Documentation
Related Tools
References
- • Castro et al. 2012. Cancer Immunol Immunother. 61(7):1005-18. PMID: 22127365.
- • Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.
- • CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.
- • Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.
- • The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
- • Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.
- • Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.
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