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Contributor Information

  • Name Lawrence Banks
  • Institute International Centre For Genetic Engineering And Biotechnology (ICGEB)

Tool Details

  • Tool name: Anti-HPV-18 E6phospho
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Reactivity: Human papilloma virus
  • Host: Rabbit
  • Application: WB
  • Description: Nearly all cases of cervical cancer are associated with HPV infection, with two types, HPV16 and HPV18, present in 70% of cases. A defining feature of cervical cancers and derived cell lines is the continued expression of two viral oncoproteins, E6 and E7. A defining characteristic of E6 oncoproteins derived from cancer-causing HPV types is the presence of a PDZ binding motif (PBM) at the extreme carboxy terminus of the protein which is absent from E6 proteins derived from the so-called low-risk HPV types. Within this PBM is also a protein kinase A (PKA) phospho-acceptor site, which is thought to negatively regulate the association of E6 with its PDZ domain-containing substrates
  • Immunogen: Peptide: RQERLQRRRETQV
  • Research area: Microbiology

  • For Research Use Only

Target Details

  • Target: HPV E6
  • Target background: Nearly all cases of cervical cancer are associated with HPV infection, with two types, HPV16 and HPV18, present in 70% of cases. A defining feature of cervical cancers and derived cell lines is the continued expression of two viral oncoproteins, E6 and E7. A defining characteristic of E6 oncoproteins derived from cancer-causing HPV types is the presence of a PDZ binding motif (PBM) at the extreme carboxy terminus of the protein which is absent from E6 proteins derived from the so-called low-risk HPV types. Within this PBM is also a protein kinase A (PKA) phospho-acceptor site, which is thought to negatively regulate the association of E6 with its PDZ domain-containing substrates

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Storage buffer: Serum
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Boon et al. 2015. J Virol. 89(3):1579-86. PMID: 25410862.
  • •  Cancer-causing human papillomavirus E6 proteins display major differences in the phospho-regulation of their PDZ interactions.
  • •  Boon et al. 2013. J Virol. 87(3):1586-95. PMID: 23175360.
  • •  High-risk human papillomavirus E6 oncoproteins interact with 14-3-3 in a PDZ binding motif-dependent manner.