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Contributor Information

  • Name Giampietro Schiavo
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Spastin [Sp 6C6]
  • Clone: Sp 6C6
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse ; Rat
  • Host: Mouse
  • Molecular weight of the target: 52 kDa
  • Application: ELISA ; IF ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody for investigation into hereditary spastic paraplegias.
  • Immunogen: Recombinant Spastin
  • Immunogen UniProt ID: Q9UBP0
  • Isotype: IgG2a
  • Research area: Tissue-specific biology; Cell biology; Cell signaling and signal transduction; Genetics; Metabolism; Neurobiology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Spastin
  • Target molecular weight: 52 kDa
  • Target background: Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Spastin is involved in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutations within spastin cause the most common form of autosomal dominant spastic paraplegia 4. Mutant forms of spastin are generally found throughout the cytoplasm rather than within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein, coded by the SPAST gene.

Application Details

  • Application: ELISA ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033.
  • •  Human spastin has multiple microtubule-related functions.