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Contributor Information

  • Name John Kemshead
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-GFAP [FD19-9] mAb
  • Alternate names: wu:fb34h11, ALXDRD, cb345, etID36982.3, FLJ42474, FLJ45472, GFAP, GFAP_HUMAN, gfapl, Glial fibrillary acidic protein, Intermediate filament protein, wu:fk42c12, xx:af56734, zgc:11485
  • Clone: FD19-9
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 50 kDa
  • Application: IHC ; IF
  • Strain: Balb/c
  • Description: Glial fibrillary acidic protein, or GFAP, is an intermediate filament (IF) protein belonging to the type III subclass of IF proteins. Like other IF proteins, GFAP is composed of an amino terminal head domain, central rod domain and a carboxy terminal tail domain. GFAP is specifically found in astroglia, a cell type which is highly responsive to neurologic insults. Astrogliosis is found to be a result of mechanical trauma, AIDS dementia, prion infection and inflammatory demylination diseases, and is accompanied by an increase in GFAP expression. GFAP is an immunohistochemical marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.
  • Immunogen: Relatively benign cerebellar astrocytoma from 5 year old child.
  • Isotype: IgG1
  • Research area: Cell biology; Cell signaling and signal transduction; Neurobiology; Stem cell biology
  • Myeloma used: P3X63Ag8

  • For Research Use Only

Target Details

  • Target: Glial fibrillary acid protein
  • Target molecular weight: 50 kDa
  • Target background: Glial fibrillary acidic protein, or GFAP, is an intermediate filament (IF) protein belonging to the type III subclass of IF proteins. Like other IF proteins, GFAP is composed of an amino terminal head domain, central rod domain and a carboxy terminal tail domain. GFAP is specifically found in astroglia, a cell type which is highly responsive to neurologic insults. Astrogliosis is found to be a result of mechanical trauma, AIDS dementia, prion infection and inflammatory demylination diseases, and is accompanied by an increase in GFAP expression. GFAP is an immunohistochemical marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.

Application Details

  • Application: IHC ; IF

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Yang et al. 2007. J Gastroenterol Hepatol. 22(9):1460-8. PMID: 17645461.
  • •  Isolation and characterization of human gastric cell lines with stem cell phenotypes.
  • •  Hagemann et al. 2006. J Neurosci Methods. 156(1-2):194-202. PMID: 16621008.
  • •  High efficiency transfection of glioma cell lines and primary cells for overexpression and RNAi experiments.
  • •  Coakham et al. 1984. Lancet. 1(8386):1095-8. PMID: 6202990.
  • •  Use of monoclonal antibody panel to identify malignant cells in cerebrospinal fluid.
  • •  Garson JA. The development and characterisation of monoclonal antibodies for use in neuropathology. MD thesis. University of Birmingham, 1983